DNTP009174
dTTP
dNTP Pool | |
---|---|
Pool identifier | POOL002295 |
Members of this Pool |
|
Members of control pools |
---|
POOL002293 |
|
Last modified: 10/Sep/2021
Results | |
---|---|
Publication |
Koc A, Mathews CK, Wheeler LJ, Gross MK, Merrill GF: Thioredoxin is required for deoxyribonucleotide pool maintenance during S phase.,
J Biol Chem, 2006
PubMed |
Value | 72.9 | Dimension | μM |
Error | Error not provided. |
Data presentation in publication | diagram |
Source | |
---|---|
Source organism |
baker's yeast
(Saccharomyces cerevisiae)
Taxonomy 4932 |
Bacterial or fungal strain | SSC18 |
Sample source | strain |
Compartment | whole cell |
Experiment Details | |
---|---|
Measurement type | dNTP incorporation assay |
Extraction method | methanol |
Remarks to measurement type | alternative extraction with freon-amide |
Growth conditions | Cells were grown in YEPD (2% bacto-yeast extract, 2% bacto-peptone, 2% glucose); synchronized; 15 min after release |
Treatment | |
---|---|
Drug/stress type | stress |
Treatment details/effects | synchronization with 100 ng/ml alpha-factor |
Genes and Proteins | |
---|---|
Gene name | CDC15 |
Gene identifier | 851274 |
Protein name | Cell division control protein 15 |
Protein identifier | P27636 |
Genetic manipulation/variation | mutation |
Mutation in protein | heat sensitive CDC15 |