Results
Publication	Koc A, Mathews CK, Wheeler LJ, Gross MK, Merrill GF: Thioredoxin is required for deoxyribonucleotide pool maintenance during S phase., J Biol Chem, 2006 PubMed
Value	16
Dimension	μM
Error	Error not provided.
Data presentation in publication	diagram

Source
Source organism	baker's yeast (Saccharomyces cerevisiae) Taxonomy 4932
Bacterial or fungal strain	MY405
Sample source	strain
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	alternative extraction with freon-amide
Growth conditions	Cells were grown in YEPD (2% bacto-yeast extract, 2% bacto-peptone, 2% glucose); synchronized; 120 min after release

Treatment
Drug/stress type	stress
Treatment details/effects	bar1 mutation facilitates alpha-factor synchronization; synchronization with 100 ng/ml alpha-factor

Genes and Proteins
Gene name	BAR1
Gene identifier	854797
Protein name	Barrierpepsin
Protein identifier	P12630
Genetic manipulation/variation	mutation
Mutation in protein	bar1 mutation
Gene name	TRX1
Gene identifier	850732
Protein name	Thioredoxin-1
Protein identifier	P22217
Genetic manipulation/variation	mutation
Mutation in protein	KO
Gene name	TRX2
Gene identifier	853123
Protein name	Thioredoxin-2
Protein identifier	P22803
Genetic manipulation/variation	mutation
Mutation in protein	KO