Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL002112
Members of this Pool
Members of control pools
POOL002108
Last modified: 10/Sep/2021
Results
Publication
Williams LN, Marjavaara L, Knowels GM, Schultz EM, Fox EJ, Chabes A, Herr AJ: dNTP pool levels modulate mutator phenotypes of error-prone DNA polymerase ε variants., Proc Natl Acad Sci U S A, 2015

PubMed PMCID
Value 123800
Dimension pmol/106 cell
Error (standard error) 4600
Data presentation in publication numerical
Source
Source organism baker's yeast (Saccharomyces cerevisiae)
Taxonomy 4932
Bacterial or fungal strain BY4733
Sample source strain
Compartment whole cell
Experiment Details
Measurement type HPLC-UV
Extraction method acid extraction
Growth conditions YPAD media (1% yeast extract, 2% bactopeptone, 0.002% adenine, 2% dextrose); 30 °C
Treatment
Treatment details/effects pol2, sml1 (RNR inhibitor protein), crt1 deletion mutant complemented with WT pol2 plasmid
Genes and Proteins
Gene name pol2
Gene identifier 855459
Protein name DNA polymerase epsilon catalytic subunit A
Protein identifier P21951
Genetic manipulation/variation mutation
Mutation in protein pol2Δ::KANMX
Gene name sml1
Gene identifier 854945
Protein name Ribonucleotide reductase inhibitor protein SML1
Protein identifier Q04964
Genetic manipulation/variation mutation
Mutation in protein sm1Δ::HIS3
Gene name crt1
Gene identifier 850873
Protein name RFX-like DNA-binding protein RFX1
Protein identifier P48743
Genetic manipulation/variation mutation
Mutation in protein crt1Δ::natMX
Gene name pol2
Gene identifier 855459
Protein name DNA polymerase epsilon catalytic subunit A
Protein identifier P21951
Genetic manipulation/variation extrachromosomal protein expression