2'-Deoxycytidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL002111
Members of this Pool
Members of control pools
POOL002108
Last modified: 10/Sep/2021
Results
Publication
Williams LN, Marjavaara L, Knowels GM, Schultz EM, Fox EJ, Chabes A, Herr AJ: dNTP pool levels modulate mutator phenotypes of error-prone DNA polymerase ε variants., Proc Natl Acad Sci U S A, 2015

PubMed PMCID
Value 9100
Dimension pmol/106 cell
Error (standard error) 800
Data presentation in publication numerical
Source
Source organism baker's yeast (Saccharomyces cerevisiae)
Taxonomy 4932
Bacterial or fungal strain BY4733
Sample source strain
Compartment whole cell
Experiment Details
Measurement type HPLC-UV
Extraction method acid extraction
Growth conditions YPAD media (1% yeast extract, 2% bactopeptone, 0.002% adenine, 2% dextrose); 30 °C
Treatment
Treatment details/effects pol2; dun1 deletion mutant strain complemented with proofreading defficient pol2-4 plasmid
Genes and Proteins
Gene name pol2
Gene identifier 855459
Protein name DNA polymerase epsilon catalytic subunit A
Protein identifier P21951
Genetic manipulation/variation mutation
Mutation in protein pol2Δ::KANMX
Gene name dun1
Gene identifier 851457
Protein name DNA damage response protein kinase DUN1
Protein identifier P39009
Genetic manipulation/variation mutation
Mutation in protein dun1Δ::TRP1
Gene name pol2
Gene identifier 855459
Protein name DNA polymerase epsilon catalytic subunit A
Protein identifier P21951
Genetic manipulation/variation extrachromosomal expression of mutant protein
Mutation in protein pol2-4