DNTP008458
dATP
dNTP Pool | |
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Pool identifier | POOL002110 |
Members of this Pool |
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Members of control pools |
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POOL002108 |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Williams LN, Marjavaara L, Knowels GM, Schultz EM, Fox EJ, Chabes A, Herr AJ: dNTP pool levels modulate mutator phenotypes of error-prone DNA polymerase ε variants.,
Proc Natl Acad Sci U S A, 2015
PubMed PMCID |
Value | 68900 | Dimension | pmol/106 cell |
Error (standard error) | 3900 |
Data presentation in publication | numerical |
Source | |
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Source organism |
baker's yeast
(Saccharomyces cerevisiae)
Taxonomy 4932 |
Bacterial or fungal strain | BY4733 |
Sample source | strain |
Compartment | whole cell |
Experiment Details | |
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Measurement type | HPLC-UV |
Extraction method | acid extraction |
Growth conditions | YPAD media (1% yeast extract, 2% bactopeptone, 0.002% adenine, 2% dextrose); 30 °C |
Treatment | |
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Treatment details/effects | pol2 deletion mutant strain complemented with polM644G plasmid (reduced polymerase accuracy) |
Genes and Proteins | |
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Gene name | pol2 |
Gene identifier | 855459 |
Protein name | DNA polymerase epsilon catalytic subunit A |
Protein identifier | P21951 |
Genetic manipulation/variation | mutation |
Mutation in protein | pol2Δ::KANMX |
Gene name | pol2 |
Gene identifier | 855459 |
Protein name | DNA polymerase epsilon catalytic subunit A |
Protein identifier | P21951 |
Genetic manipulation/variation | extrachromosomal expression of mutant protein |
Mutation in protein | polM644G |