DNTP008449
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL002108 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Williams LN, Marjavaara L, Knowels GM, Schultz EM, Fox EJ, Chabes A, Herr AJ: dNTP pool levels modulate mutator phenotypes of error-prone DNA polymerase ε variants.,
Proc Natl Acad Sci U S A, 2015
PubMed PMCID |
| Value | 35400 | Dimension | pmol/106 cell |
| Error (standard error) | 1300 |
| Data presentation in publication | numerical |
| Source | |
|---|---|
| Source organism |
baker's yeast
(Saccharomyces cerevisiae)
Taxonomy 4932 |
| Bacterial or fungal strain | BY4733 |
| Sample source | strain |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | HPLC-UV |
| Extraction method | acid extraction |
| Growth conditions | YPAD media (1% yeast extract, 2% bactopeptone, 0.002% adenine, 2% dextrose); 30 °C |
| Treatment | |
|---|---|
| Treatment details/effects | pol2 deletion mutant strain complemented with wt pol2 plasmid |
| Genes and Proteins | |
|---|---|
| Gene name | pol2 |
| Gene identifier | 855459 |
| Protein name | DNA polymerase epsilon catalytic subunit A |
| Protein identifier | P21951 |
| Genetic manipulation/variation | mutation |
| Mutation in protein | pol2Δ::KANMX |
| Gene name | pol2 |
| Gene identifier | 855459 |
| Protein name | DNA polymerase epsilon catalytic subunit A |
| Protein identifier | P21951 |
| Genetic manipulation/variation | extrachromosomal protein expression |