Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
POOL002085
Last modified: 10/Sep/2021
Results
Publication
Angulo-Ibáñez M, Rovira-Clavé X, Granados-Jaén A, Downs B, Kim YC, Wang SM, Reina M, Espel E: Erk5 contributes to maintaining the balance of cellular nucleotide levels and erythropoiesis., Cell Cycle, 2015

PubMed PMCID
Value 1214.9533
Dimension %
Error (standard deviation) 841.1215
Relative compared to dCTP
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type T-cell acute lymphoblastic leukemia
CLO Cell line name Jurkat, Clone E6-1 cell (CLO:0007045)
Cell line/type as in publication Jurkat E6.1
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type Mathews and Wheeler
Growth conditions 18 h treatment with 2 mM hydroxyurea
Treatment
Drug/stress type drug
PubChem 3657
Applied drug hydroxyurea
Treatment details/effects MAPK7 (equals Erk5) activity has been associated with cell cycle progression; hydroxyurea (HU) is a ribonucleotide reductase (RNR) inhibitor that causes depletion of dNTP pools
Genes and Proteins
Gene name MAPK7
Gene identifier 5598
Protein name Mitogen-activated protein kinase 7
Protein identifier Q13164
Genetic manipulation/variation silencing