DNTP008291
dGTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL002068 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Angulo-Ibáñez M, Rovira-Clavé X, Granados-Jaén A, Downs B, Kim YC, Wang SM, Reina M, Espel E: Erk5 contributes to maintaining the balance of cellular nucleotide levels and erythropoiesis.,
Cell Cycle, 2015
PubMed PMCID |
| Value | 0.4553 | Dimension | fold change |
| Error (standard deviation) | 0.2685 |
| Relative compared to | asynchronous control cells |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Tissue type | T-cell acute lymphoblastic leukemia |
| CLO Cell line name | Jurkat, Clone E6-1 cell (CLO:0007045) |
| Cell line/type as in publication | Jurkat E6.1 |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | Mathews and Wheeler |
| Growth conditions | 18 h treatment with 2 mM hydroxyurea |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 3657 |
| Applied drug | hydroxyurea |
| Treatment details/effects | MAPK7 (equals Erk5) activity has been associated with cell cycle progression; hydroxyurea (HU) is a ribonucleotide reductase (RNR) inhibitor that causes depletion of dNTP pools |
| Genes and Proteins | |
|---|---|
| Gene name | MAPK7 |
| Gene identifier | 5598 |
| Protein name | Mitogen-activated protein kinase 7 |
| Protein identifier | Q13164 |
| Genetic manipulation/variation | silencing |