DNTP007765
dTTP
| dNTP Pool | |
|---|---|
| This entry does not belong to any pool in the database. |
| Members of control pools |
|---|
| False |
|
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Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Pontarin G, Ferraro P, Rampazzo C, Kollberg G, Holme E, Reichard P, Bianchi V: Deoxyribonucleotide metabolism in cycling and resting human fibroblasts with a missense mutation in p53R2, a subunit of ribonucleotide reductase.,
J Biol Chem, 2011
PubMed PMCID |
| Value | 10.34 | Dimension | pmol/106 cell |
| Error (standard deviation) | 1.58 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Tissue type | skin fibroblast |
| Cell line/type as in publication | p53R2-mutated human skin fibroblasts were isolated from a patient carrying a homozygous missense mutation in the RRMB2 gene |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | modified Sherman and Fyfe; Ferraro |
| Growth conditions | Contact-inhibited cells were maintained for 7 days in MEM + 0.1% dialyzed FCS. |
| Treatment | |
|---|---|
| Treatment details/effects | ability to reduce ribonucleotides was strongly curtailed |
| Genes and Proteins | |
|---|---|
| Gene name | RRM2B |
| Gene identifier | 50484 |
| Protein name | Ribonucleoside-diphosphate reductase subunit M2 B |
| Protein identifier | Q7LG56 |
| Genetic manipulation/variation | genetic variation |
| Mutation in protein | homozygous missense mutation |