Results
Publication	Pontarin G, Ferraro P, Rampazzo C, Kollberg G, Holme E, Reichard P, Bianchi V: Deoxyribonucleotide metabolism in cycling and resting human fibroblasts with a missense mutation in p53R2, a subunit of ribonucleotide reductase., J Biol Chem, 2011 PubMed PMCID
Value	1.12
Dimension	pmol/106 cell
Error	Error not provided.
Data presentation in publication	diagram

Source
Source organism	human (Homo sapiens) Taxonomy 9606
Sample source	cell line
Tissue type	skin fibroblast
Cell line/type as in publication	p53R2-mutated human skin fibroblasts were isolated from a patient carrying a homozygous missense mutation in the RRMB2 gene
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	modified Sherman and Fyfe; Ferraro
Growth conditions	grown in MEM + 10% FCS for 10 days to reach confluence; Incubation continued in MEM + 0.1% dialyzed FCS for 18 days

Treatment
Treatment details/effects	ability to reduce ribonucleotides was strongly curtailed

Genes and Proteins
Gene name	RRM2B
Gene identifier	50484
Protein name	Ribonucleoside-diphosphate reductase subunit M2 B
Protein identifier	Q7LG56
Genetic manipulation/variation	genetic variation
Mutation in protein	homozygous missense mutation