Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001927
Members of this Pool
Last modified: 10/Sep/2021
Results
Publication
Pontarin G, Ferraro P, Rampazzo C, Kollberg G, Holme E, Reichard P, Bianchi V: Deoxyribonucleotide metabolism in cycling and resting human fibroblasts with a missense mutation in p53R2, a subunit of ribonucleotide reductase., J Biol Chem, 2011

PubMed PMCID
Value 2.56
Dimension pmol/106 cell
Error (standard deviation) 0.32
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type skin fibroblast
CLO Cell line name immortal human skin-derived fibroblast cell line cell (CLO:0000437)
Cell line/type as in publication control skin fibroblast
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type modified Sherman and Fyfe; Ferraro
Growth conditions grown in MEM + 10% FCS for 10 days to reach confluence; Incubation continued in MEM + 0.1% dialyzed FCS for 27 days
Treatment
There was no treatment in this experiment.
Genes and Proteins
No genes or proteins manipulated.