DNTP007719
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL001925 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Pontarin G, Ferraro P, Rampazzo C, Kollberg G, Holme E, Reichard P, Bianchi V: Deoxyribonucleotide metabolism in cycling and resting human fibroblasts with a missense mutation in p53R2, a subunit of ribonucleotide reductase.,
J Biol Chem, 2011
PubMed PMCID |
| Value | 2.32 | Dimension | pmol/106 cell |
| Error (standard deviation) | 0.53 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Tissue type | skin fibroblast |
| CLO Cell line name | immortal human skin-derived fibroblast cell line cell (CLO:0000437) |
| Cell line/type as in publication | control skin fibroblast |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | modified Sherman and Fyfe; Ferraro |
| Growth conditions | grown in MEM + 10% FCS for 10 days to reach confluence; Incubation continued in MEM + 0.1% dialyzed FCS for 10 days |
| Treatment | |
|---|---|
| There was no treatment in this experiment. |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |