2'-Deoxycytidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001920
Members of this Pool
Members of control pools
POOL001893
Last modified: 10/Sep/2021
Results
Publication
Pontarin G, Ferraro P, Bee L, Reichard P, Bianchi V: Mammalian ribonucleotide reductase subunit p53R2 is required for mitochondrial DNA replication and DNA repair in quiescent cells., Proc Natl Acad Sci U S A, 2012

PubMed PMCID
Value 5.63
Dimension pmol/106 cell
Error Error not provided.
Data presentation in publication numerical
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type fibroblasts
Cell line/type as in publication p53R2 mutant fibroblast
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type modified Sherman and Fyfe; Ferraro
Growth conditions UV irradiation (12 J/m2); 5 μM of CdR; 5 μM of GdR; cells kept in low serum before e x posure for 7 days
Treatment
Drug/stress type drug + stress
Stress type UV
PubChem 13711 , 135398592
Applied drug deoxycytidine, deoxyguanosine
Treatment details/effects UV (DNA damage); ability to reduce ribonucleotides was strongly curtailed
Genes and Proteins
Gene name RRM2B
Gene identifier 50484
Protein name Ribonucleoside-diphosphate reductase subunit M2 B
Protein identifier Q7LG56
Genetic manipulation/variation mutation
Mutation in protein homozygous missense mutation in the iron-binding center