Results
Publication	Rampazzo C, Fabris S, Franzolin E, Crovatto K, Frangini M, Bianchi V: Mitochondrial thymidine kinase and the enzymatic network regulating thymidine triphosphate pools in cultured human cells., J Biol Chem, 2007 PubMed
Value	1
Dimension	pmol/106 cell
Error (standard deviation)	0.02
Data presentation in publication	diagram

Source
Source organism	human (Homo sapiens) Taxonomy 9606
Sample source	cell line
Tissue type	skin fibroblast
Cell line/type as in publication	C63
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	modified Sherman and Fyfe; Ferraro
Growth conditions	quiescent cultures transfected for 10 days, then medium repleaced with fresh medium with 0.1% dialyzed serum

Treatment
Drug/stress type	drug
PubChem	3657 , 5802 , 5789
Applied drug	hydroxyurea, 5-bromouracil;3H-deoxythymidine isotope
Treatment details/effects	3 mM HU; 100 μM BU; [3H]TdR: 20 min

Genes and Proteins
Gene name	TK2
Gene identifier	7084
Protein name	Thymidine kinase 2, mitochondrial
Protein identifier	O00142
Genetic manipulation/variation	silencing