Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
N/A
Last modified: 10/Sep/2021
Results
Publication
Rampazzo C, Fabris S, Franzolin E, Crovatto K, Frangini M, Bianchi V: Mitochondrial thymidine kinase and the enzymatic network regulating thymidine triphosphate pools in cultured human cells., J Biol Chem, 2007

PubMed
Value 0.68
Dimension pmol/106 cell
Error (standard deviation) 0.02
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type skin fibroblast
Cell line/type as in publication C63
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type modified Sherman and Fyfe; Ferraro
Growth conditions quiescent cultures transfected for 10 days, then medium repleaced with fresh medium with 0.1% dialyzed serum
Treatment
Drug/stress type drug
PubChem 3657 , 5789
Applied drug hydroxyurea, 3H-deoxythymidine isotope
Treatment details/effects 3 mM HU; [3H]TdR: 5 min
Genes and Proteins
Gene name TK2
Gene identifier 7084
Protein name Thymidine kinase 2, mitochondrial
Protein identifier O00142
Genetic manipulation/variation silencing