DNTP007517
dTTP
| dNTP Pool | |
|---|---|
| This entry does not belong to any pool in the database. |
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Rampazzo C, Fabris S, Franzolin E, Crovatto K, Frangini M, Bianchi V: Mitochondrial thymidine kinase and the enzymatic network regulating thymidine triphosphate pools in cultured human cells.,
J Biol Chem, 2007
PubMed |
| Value | 1.8 | Dimension | pmol/106 cell |
| Error (standard deviation) | 0.08 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Tissue type | skin fibroblast |
| Cell line/type as in publication | C63 |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | modified Sherman and Fyfe; Ferraro |
| Growth conditions | quiescent cultures transfected for 10 days, then medium repleaced with fresh medium with 0.1% dialyzed serum |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 3657 , 5789 |
| Applied drug | hydroxyurea, 3H-deoxythymidine isotope |
| Treatment details/effects | 3 mM HU; [3H]TdR: 20 min; transfection control |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |