Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
N/A N/A
Last modified: 10/Sep/2021
Results
Publication
Rampazzo C, Fabris S, Franzolin E, Crovatto K, Frangini M, Bianchi V: Mitochondrial thymidine kinase and the enzymatic network regulating thymidine triphosphate pools in cultured human cells., J Biol Chem, 2007

PubMed
Value 0.8197
Dimension pmol/106 cell
Error Error not provided.
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type skin fibroblast
Cell line/type as in publication C63
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type modified Sherman and Fyfe; Ferraro
Growth conditions 100 nM [3H]TdR in cultures of C63 fibroblasts maintained for 10 days in 0.1% FCS after reaching confluence before treatment
Treatment
Drug/stress type drug
PubChem 5789 , 3657
Applied drug 3H-deoxythymidine isotope, hydroxyurea
Treatment details/effects Culture received a 60-min pulse with [3H]TdR, 15 mM HU was added at 50 min of the pulse, followed by a 180-min chase without TdR in the medium
Genes and Proteins
No genes or proteins manipulated.