DNTP007499
dTTP
dNTP Pool | |
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This entry does not belong to any pool in the database. |
Members of control pools | |
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N/A | N/A |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Rampazzo C, Fabris S, Franzolin E, Crovatto K, Frangini M, Bianchi V: Mitochondrial thymidine kinase and the enzymatic network regulating thymidine triphosphate pools in cultured human cells.,
J Biol Chem, 2007
PubMed |
Value | 1.2064 | Dimension | pmol/106 cell |
Error | Error not provided. |
Data presentation in publication | diagram |
Source | |
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Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
Sample source | cell line |
Tissue type | skin fibroblast |
Cell line/type as in publication | C63 |
Compartment | whole cell |
Experiment Details | |
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Measurement type | dNTP incorporation assay |
Extraction method | methanol |
Remarks to measurement type | modified Sherman and Fyfe; Ferraro |
Growth conditions | 100 nM [3H]TdR in cultures of C63 fibroblasts maintained for 10 days in 0.1% FCS after reaching confluence before treatment |
Treatment | |
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Drug/stress type | drug |
PubChem | 5789 , 3657 |
Applied drug | 3H-deoxythymidine isotope, hydroxyurea |
Treatment details/effects | Culture received a 60-min pulse with [3H]TdR, 3 mM HU was added at 50 min of the pulse, followed by a 5-min chase without TdR in the medium |
Genes and Proteins | |
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No genes or proteins manipulated. | |