DNTP007493
dTTP
| dNTP Pool | |
|---|---|
| This entry does not belong to any pool in the database. |
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Rampazzo C, Fabris S, Franzolin E, Crovatto K, Frangini M, Bianchi V: Mitochondrial thymidine kinase and the enzymatic network regulating thymidine triphosphate pools in cultured human cells.,
J Biol Chem, 2007
PubMed |
| Value | 1.6469 | Dimension | pmol/106 cell |
| Error | Error not provided. |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Tissue type | skin fibroblast |
| Cell line/type as in publication | C63 |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | modified Sherman and Fyfe; Ferraro |
| Growth conditions | 100 nM [3H]TdR in cultures of C63 fibroblasts maintained for 10 days in 0.1% FCS after reaching confluence before treatment |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 5789 |
| Applied drug | 3H-deoxythymidine isotope |
| Treatment details/effects | Culture received a 60-min pulse with [3H]TdR followed by a 5-min chase without TdR in the medium |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |