DNTP007424
dTTP
| dNTP Pool | |
|---|---|
| This entry does not belong to any pool in the database. |
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Frangini M, Rampazzo C, Franzolin E, Lara MC, Vilà MR, Martí R, Bianchi V: Unchanged thymidine triphosphate pools and thymidine metabolism in two lines of thymidine kinase 2-mutated fibroblasts.,
FEBS J, 2009
PubMed |
| Value | 2.29 | Dimension | pmol/106 cell |
| Error | Error not provided. |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Tissue type | fibroblasts |
| CLO Cell line name | immortal myoblast cell line cell (CLO:0000057) |
| Cell line/type as in publication | Control fibroblast |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | modified Sherman and Fyfe; Ferraro |
| Growth conditions | Quiescent cultures were incubated for 60 min with 100 nM [3H]thymidine; Cultures shifted to 100 nM nonradioactive thymidine medium for 5 min |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| Applied drug | [3H]thymidine isotope |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |