Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
N/A
Last modified: 10/Sep/2021
Results
Publication
Frangini M, Rampazzo C, Franzolin E, Lara MC, Vilà MR, Martí R, Bianchi V: Unchanged thymidine triphosphate pools and thymidine metabolism in two lines of thymidine kinase 2-mutated fibroblasts., FEBS J, 2009

PubMed
Value 2.42
Dimension pmol/106 cell
Error (standard deviation) 0.4
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type fibroblasts
Cell line/type as in publication TK2‐mutated fibroblast line, derived from a 10 year old patients with different compound heterozygous genotypes: T77M/R161K
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type modified Sherman and Fyfe; Ferraro
Growth conditions Quiescent culture, incubated for 20 min with 25 nM [3H]thymidine
Treatment
Drug/stress type drug
Applied drug [3H]thymidine isotope
Treatment details/effects Mitochondrial thymidine kinase (TK2) deficiency
Genes and Proteins
Gene name TK2
Gene identifier 7084
Protein name Thymidine kinase 2, mitochondrial
Protein identifier O00142
Genetic manipulation/variation genetic variation
Mutation in protein heterozygous genotypes, T77M/R161K