DNTP007405
dTTP
dNTP Pool | |
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This entry does not belong to any pool in the database. |
Members of control pools |
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N/A |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Frangini M, Rampazzo C, Franzolin E, Lara MC, Vilà MR, Martí R, Bianchi V: Unchanged thymidine triphosphate pools and thymidine metabolism in two lines of thymidine kinase 2-mutated fibroblasts.,
FEBS J, 2009
PubMed |
Value | 1.33 | Dimension | pmol/106 cell |
Error (standard deviation) | 0.28 |
Data presentation in publication | diagram |
Source | |
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Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
Sample source | cell line |
Tissue type | fibroblasts |
CLO Cell line name | immortal myoblast cell line cell (CLO:0000057) |
Cell line/type as in publication | Control fibroblast |
Compartment | whole cell |
Experiment Details | |
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Measurement type | dNTP incorporation assay |
Extraction method | methanol |
Remarks to measurement type | modified Sherman and Fyfe; Ferraro |
Growth conditions | Quiescent cultures of control (Ca) fibroblasts were incubated for 5 min with 25 nM [3H]thymidine; incubated in the presence of 1 μM BVDU added 15 min before [3H]thymidine, and the incubation was continued for 5 min |
Treatment | |
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Drug/stress type | drug |
PubChem | 6438404 |
Applied drug | 5‐bromovinyl‐2‐deoxyuridine, [3H]thymidine isotope |
Genes and Proteins | |
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No genes or proteins manipulated. | |