Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
N/A
Last modified: 10/Sep/2021
Results
Publication
Frangini M, Rampazzo C, Franzolin E, Lara MC, Vilà MR, Martí R, Bianchi V: Unchanged thymidine triphosphate pools and thymidine metabolism in two lines of thymidine kinase 2-mutated fibroblasts., FEBS J, 2009

PubMed
Value 1.33
Dimension pmol/106 cell
Error (standard deviation) 0.28
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type fibroblasts
CLO Cell line name immortal myoblast cell line cell (CLO:0000057)
Cell line/type as in publication Control fibroblast
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type modified Sherman and Fyfe; Ferraro
Growth conditions Quiescent cultures of control (Ca) fibroblasts were incubated for 5 min with 25 nM [3H]thymidine; incubated in the presence of 1 μM BVDU added 15 min before [3H]thymidine, and the incubation was continued for 5 min
Treatment
Drug/stress type drug
PubChem 6438404
Applied drug 5‐bromovinyl‐2‐deoxyuridine, [3H]thymidine isotope
Genes and Proteins
No genes or proteins manipulated.