Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001839
Members of this Pool
Last modified: 10/Sep/2021
Results
Publication
Ferraro P, Pontarin G, Crocco L, Fabris S, Reichard P, Bianchi V: Mitochondrial deoxynucleotide pools in quiescent fibroblasts: a possible model for mitochondrial neurogastrointestinal encephalomyopathy (MNGIE)., J Biol Chem, 2005

PubMed
Value 6.54
Dimension pmol/106 cell
Error (standard error) -1.82; +3.47
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type lung fibroblasts
Cell line/type as in publication lung fibroblasts (CCD34Lu)
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type modified Sherman and Fyfe; Ferraro
Growth conditions Quiescent cells; contact-inhibited lung fibroblasts in 0.1% FCS+10–40 μM thymidine + deoxyuridine; Averaged value from the four time points: 13, 23, 34, 41 days
Treatment
Drug/stress type drug
PubChem 13712 , 5789
Applied drug deoxyuridine, thymidine
Genes and Proteins
No genes or proteins manipulated.