Results
Publication	Franzolin E, Pontarin G, Rampazzo C, Miazzi C, Ferraro P, Palumbo E, Reichard P, Bianchi V: The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells., Proc Natl Acad Sci U S A, 2013 PubMed PMCID
Value	64.14
Dimension	pmol/106 cell
Error	Error not provided.
Data presentation in publication	diagram

Source
Source organism	human (Homo sapiens) Taxonomy 9606
Sample source	cell line
Tissue type	skin fibroblast
CLO Cell line name	immortal human skin-derived fibroblast cell line cell (CLO:0000437)
Cell line/type as in publication	Human control skin fibroblast cell line immortalized with human telomerase coded by plasmid CMV–hTERT/PGK–Puro
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	modified Sherman and Fyfe; Ferraro
Growth conditions	3 day recovery period after siRNA silencing of SMAHD1

Treatment
Treatment details/effects	SAMHD1 silencing

Genes and Proteins
Gene name	SAMHD1
Gene identifier	25939
Protein name	Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Protein identifier	Q9X3Z3
Genetic manipulation/variation	silencing