2'-Deoxycytidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001799
Members of this Pool
Last modified: 10/Sep/2021
Results
Publication
Franzolin E, Pontarin G, Rampazzo C, Miazzi C, Ferraro P, Palumbo E, Reichard P, Bianchi V: The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells., Proc Natl Acad Sci U S A, 2013

PubMed PMCID
Value 32.17
Dimension pmol/106 cell
Error Error not provided.
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type skin fibroblast
CLO Cell line name immortal human skin-derived fibroblast cell line cell (CLO:0000437)
Cell line/type as in publication Human control skin fibroblast cell line immortalized with human telomerase coded by plasmid CMV–hTERT/PGK–Puro
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type modified Sherman and Fyfe; Ferraro
Growth conditions Silencing: AllStars Negative Control siRNA (Quiagen)
Treatment
Drug/stress type stress
Treatment details/effects Control si-RNA: AllStars Negative Control siRNA (Qiagen)
Genes and Proteins
No genes or proteins manipulated.