DNTP007094
dGTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL001783 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Franzolin E, Pontarin G, Rampazzo C, Miazzi C, Ferraro P, Palumbo E, Reichard P, Bianchi V: The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells.,
Proc Natl Acad Sci U S A, 2013
PubMed PMCID |
| Value | 0.4 | Dimension | pmol/106 cell |
| Error (standard error of the mean) | 0.1 |
| Data presentation in publication | numerical |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Tissue type | P53R2- skin fibroblasts |
| Cell line/type as in publication | skin fibroblast cell line derived from a patient with an inactivating mutation in RRM2B, the gene for p53r2 |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | modified Sherman and Fyfe; Ferraro |
| Growth conditions | Quiescent cultures; 10 d of serum starvation |
| Treatment | |
|---|---|
| Drug/stress type | stress |
| Stress type | starvation |
| Treatment details/effects | Starvation; transfection with control si-RNA |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |