DNTP007093
dCTP
dNTP Pool | |
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Pool identifier | POOL001782 |
Members of this Pool |
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Members of control pools |
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POOL001781 |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Franzolin E, Pontarin G, Rampazzo C, Miazzi C, Ferraro P, Palumbo E, Reichard P, Bianchi V: The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells.,
Proc Natl Acad Sci U S A, 2013
PubMed PMCID |
Value | 5 | Dimension | pmol/106 cell |
Error (standard error of the mean) | 0.9 |
Data presentation in publication | numerical |
Source | |
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Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
Sample source | cell line |
Tissue type | skin fibroblast |
CLO Cell line name | immortal human skin-derived fibroblast cell line cell (CLO:0000437) |
Cell line/type as in publication | Human control skin fibroblast cell line immortalized with human telomerase coded by plasmid CMV–hTERT/PGK–Puro |
Compartment | whole cell |
Experiment Details | |
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Measurement type | dNTP incorporation assay |
Extraction method | methanol |
Remarks to measurement type | modified Sherman and Fyfe; Ferraro |
Growth conditions | Quiescent cultures; 10 d of serum starvation |
Treatment | |
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Drug/stress type | stress |
Stress type | starvation |
Treatment details/effects | Starvation |
Genes and Proteins | |
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Gene name | SAMHD1 |
Gene identifier | 25939 |
Protein name | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 |
Protein identifier | Q9X3Z3 |
Genetic manipulation/variation | silencing |