2'-Deoxyadenosine 5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001782
Members of this Pool
Members of control pools
POOL001781
Last modified: 10/Sep/2021
Results
Publication
Franzolin E, Pontarin G, Rampazzo C, Miazzi C, Ferraro P, Palumbo E, Reichard P, Bianchi V: The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells., Proc Natl Acad Sci U S A, 2013

PubMed PMCID
Value 4.5
Dimension pmol/106 cell
Error (standard error of the mean) 1
Data presentation in publication numerical
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type skin fibroblast
CLO Cell line name immortal human skin-derived fibroblast cell line cell (CLO:0000437)
Cell line/type as in publication Human control skin fibroblast cell line immortalized with human telomerase coded by plasmid CMV–hTERT/PGK–Puro
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type modified Sherman and Fyfe; Ferraro
Growth conditions Quiescent cultures; 10 d of serum starvation
Treatment
Drug/stress type stress
Stress type starvation
Treatment details/effects Starvation
Genes and Proteins
Gene name SAMHD1
Gene identifier 25939
Protein name Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Protein identifier Q9X3Z3
Genetic manipulation/variation silencing