DNTP007024
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL001765 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Franzolin E, Pontarin G, Rampazzo C, Miazzi C, Ferraro P, Palumbo E, Reichard P, Bianchi V: The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells.,
Proc Natl Acad Sci U S A, 2013
PubMed PMCID |
| Value | 84.4 | Dimension | pmol/106 cell |
| Error | Error not provided. |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Tissue type | lung fibroblasts |
| Cell line/type as in publication | lung fibroblasts (CCD34Lu) |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | modified Sherman and Fyfe; Ferraro |
| Growth conditions | silencing: siSAMHD1– 3 siRNA; 27% of the cells at S phase |
| Treatment |
|---|
| Genes and Proteins | |
|---|---|
| Gene name | SAMHD1 |
| Gene identifier | 25939 |
| Protein name | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 |
| Protein identifier | Q9X3Z3 |
| Genetic manipulation/variation | silencing |