2'-Deoxyguanosine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001762
Members of this Pool
Members of control pools
POOL001760
Last modified: 10/Sep/2021
Results
Publication
Franzolin E, Pontarin G, Rampazzo C, Miazzi C, Ferraro P, Palumbo E, Reichard P, Bianchi V: The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells., Proc Natl Acad Sci U S A, 2013

PubMed PMCID
Value 30.3
Dimension pmol/106 cell
Error (standard error of the mean) 2.9
Data presentation in publication numerical
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type skin fibroblast
CLO Cell line name immortal human skin-derived fibroblast cell line cell (CLO:0000437)
Cell line/type as in publication Human control skin fibroblast cell line immortalized with human telomerase coded by plasmid CMV–hTERT/PGK–Puro
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type modified Sherman and Fyfe; Ferraro
Growth conditions transfected for 48 h with anti SAMHD1 si-RNA
Treatment
Genes and Proteins
Gene name SAMHD1
Gene identifier 25939
Protein name Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Protein identifier Q9X3Z3
Genetic manipulation/variation silencing