DNTP007002
dGTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL001760 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Franzolin E, Pontarin G, Rampazzo C, Miazzi C, Ferraro P, Palumbo E, Reichard P, Bianchi V: The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells.,
Proc Natl Acad Sci U S A, 2013
PubMed PMCID |
| Value | 12.3 | Dimension | pmol/106 cell |
| Error (standard error of the mean) | 1.4 |
| Data presentation in publication | numerical |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Tissue type | skin fibroblast |
| CLO Cell line name | immortal human skin-derived fibroblast cell line cell (CLO:0000437) |
| Cell line/type as in publication | Human control skin fibroblast cell line immortalized with human telomerase coded by plasmid CMV–hTERT/PGK–Puro |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | modified Sherman and Fyfe; Ferraro |
| Growth conditions | transfected for 48 h with control si-RNA: AllStars Negative Control siRNA (Qiagen) |
| Treatment | |
|---|---|
| Drug/stress type | stress |
| Treatment details/effects | Control si-RNA: AllStars Negative Control siRNA (Qiagen) |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |