DNTP005917 (dCTP)

DNTP005918

dATP

DNTP005919 (dGTP)

2'-Deoxyadenosine 5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001478
Members of this Pool
Members of control pools
POOL001479
Last modified: 10/Sep/2021
Results
Publication
Pfister SX, Markkanen E, Jiang Y, Sarkar S, Woodcock M, Orlando G, Mavrommati I, Pai CC, Zalmas LP, Drobnitzky N, Dianov GL, Verrill C, Macaulay VM, Ying S, La Thangue NB, D'Angiolella V, Ryan AJ, Humphrey TC: Inhibiting WEE1 Selectively Kills Histone H3K36me3-Deficient Cancers by dNTP Starvation., Cancer Cell, 2015

PubMed PMCID
Value 1.1814
Dimension fold change
Error (standard error of the mean) 0.1581
Relative compared to control
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
Tissue type human osteosarcoma
CLO Cell line name U-2 OS cell (CLO:0009454)
Cell line/type as in publication U-2 OS
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type Sherman and Fyfe
Growth conditions U2OS cells stably expressing a degradation-resistant mutant RRM2 (T33A), transfected with SETD2 siRNA (siSETD2) (48 hr) prior to treatment with AZD1775 (200 nM) (24 hr).
Treatment
Drug/stress type drug
PubChem 24856436
Applied drug AZD1775
Treatment details/effects drug treatment; stable expression of degradation-resistant mutant RRM2 (T33A); silencing
Genes and Proteins
Gene name RRM2
Gene identifier 6241
Protein name Ribonucleoside-diphosphate reductase subunit M2
Protein identifier P31350
Genetic manipulation/variation extrachromosomal expression of mutant protein
Mutation in protein degradation-resistant mutant RRM2 (T33A)
Gene name SETD2
Gene identifier 29072
Protein name Histone-lysine N-methyltransferase SETD2
Protein identifier Q9BYW2
Genetic manipulation/variation silencing