DNTP005912
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL001477 |
| Members of this Pool |
|
| Members of control pools |
|---|
| POOL001479 |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Pfister SX, Markkanen E, Jiang Y, Sarkar S, Woodcock M, Orlando G, Mavrommati I, Pai CC, Zalmas LP, Drobnitzky N, Dianov GL, Verrill C, Macaulay VM, Ying S, La Thangue NB, D'Angiolella V, Ryan AJ, Humphrey TC: Inhibiting WEE1 Selectively Kills Histone H3K36me3-Deficient Cancers by dNTP Starvation.,
Cancer Cell, 2015
PubMed PMCID |
| Value | 1.0791 | Dimension | fold change |
| Error (standard error of the mean) | 0.0186 |
| Relative compared to | control |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Tissue type | human osteosarcoma |
| CLO Cell line name | U-2 OS cell (CLO:0009454) |
| Cell line/type as in publication | U-2 OS |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | Sherman and Fyfe |
| Growth conditions | U2OS cells stably expressing a degradation-resistant mutant RRM2 (T33A), transfected with SETD2 siRNA (siSETD2) (48 hr) prior to treatment with DMSO (24 hr). |
| Treatment | |
|---|---|
| Treatment details/effects | vehicle control (DMSO); stable expression of degradation-resistant mutant RRM2 (T33A); silencing |
| Genes and Proteins | |
|---|---|
| Gene name | RRM2 |
| Gene identifier | 6241 |
| Protein name | Ribonucleoside-diphosphate reductase subunit M2 |
| Protein identifier | P31350 |
| Genetic manipulation/variation | extrachromosomal expression of mutant protein |
| Mutation in protein | degradation-resistant mutant RRM2 (T33A) |
| Gene name | SETD2 |
| Gene identifier | 29072 |
| Protein name | Histone-lysine N-methyltransferase SETD2 |
| Protein identifier | Q9BYW2 |
| Genetic manipulation/variation | silencing |