2'-Deoxycytidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001456
Members of this Pool
Members of control pools
POOL001453
Last modified: 10/Sep/2021
Results
Publication
Ren Q, Kao V, Grem JL: Cytotoxicity and DNA fragmentation associated with sequential gemcitabine and 5-fluoro-2'-deoxyuridine in HT-29 colon cancer cells., Clin Cancer Res, 1998

PubMed
Value 43.9
Dimension pmol/106 cell
Error (standard error) 8.7
Data presentation in publication numerical
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
CLO Cell line name HT-29 cell (CLO:0004283)
Cell line/type as in publication HT-29
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method acid extraction
Remarks to measurement type Sherman and Fyfe
Growth conditions RPMI 1640 with 10% FBS and 2 mM glutamine; cells were treated with 15 nM dFdCyd (gemcitabine) for 4 hours, then 10 nM FdUrd (floxuridine) for 4 hours
Treatment
Drug/stress type drug
PubChem 60750 , 5790
Applied drug gemcitabine, floxuridine
Treatment details/effects cells were treated with gemcitabine, a deoxycytidine analogue with antineoplastic activity; cells were treated with floxuridine, a pyrimidine monophosphate analogue with antineoplastic activity
Genes and Proteins
No genes or proteins manipulated.