Results
Publication	Delfarah A, Parrish S, Junge JA, Yang J, Seo F, Li S, Mac J, Wang P, Fraser SE, Graham NA: Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cells., J Biol Chem, 2019 PubMed PMCID
Value	155.25
Dimension	%
Error (standard deviation)	26.07
Relative compared to	control
Data presentation in publication	diagram

Source
Source organism	human (Homo sapiens) Taxonomy 9606
Sample source	primary cells
Cell line/type as in publication	human mammary epithelial cells (HMECs)
Compartment	whole cell

Experiment Details
Measurement type	LC-MS
Extraction method	methanol
Growth conditions	cells were cultured in M87A medium and after 14 population doublings infected with pLentiPGK-hygro encoding human telomerase, then selected with hygromycin and maintained for 45 days (senescent stage)

Treatment
Treatment details/effects	expression of hTERT, essential for the replication of chromosome termini

Genes and Proteins
Gene name	hTERT
Gene identifier	9606
Protein name	telomerase reverse transcriptase
Protein identifier	O94807
Genetic manipulation/variation	extrachromosomal protein expression