DNTP005718
dTTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL001434 |
| Members of this Pool |
|
| Members of control pools | |
|---|---|
| POOL001433 | POOL001432 |
|
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Ingraham HA, Dickey L, Goulian M: DNA fragmentation and cytotoxicity from increased cellular deoxyuridylate.,
Biochemistry, 1986
PubMed |
| Value | 30 | Dimension | pmol/106 cell |
| Error | Error not provided. |
| Data presentation in publication | numerical |
| Source | |
|---|---|
| Source organism |
Chinese hamster
(Cricetulus griseus)
Taxonomy 10029 |
| Sample source | cell line |
| Cell line/type as in publication | CCL39 |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | acid extraction |
| Remarks to measurement type | Solter and Handschumacher |
| Growth conditions | modified Eagle's medium with 10% FBS, 100 μM hypoxanthine, 10 μM methotrexate and 5 μM thymidine |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| PubChem | 126941 |
| Applied drug | methotrexate |
| Treatment details/effects | Dihydrofolate reductase inhibition |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |