Results
Publication	Martínez-Arribas B, Requena CE, Pérez-Moreno G, Ruíz-Pérez LM, Vidal AE, González-Pacanowska D: DCTPP1 prevents a mutator phenotype through the modulation of dCTP, dTTP and dUTP pools., Cell Mol Life Sci, 2020 PubMed PMCID
Value	0.01
Dimension	pmol/106 cell
Error (standard deviation)	0.02
Data presentation in publication	diagram

Source
Source organism	human (Homo sapiens) Taxonomy 9606
Sample source	cell line
Cell line/type as in publication	DCTPP1-knockout derivative of HAP1
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	Sherman and Fyfe
Growth conditions	IMDM supplemented with 10% FBS and antibiotics; cells were transfected with TK1 silencing siRNA

Treatment
Treatment details/effects	lack of functional dCTP pyrophosphatase 1, which hydrolyzes dNTPs to the corresponding nucleoside monophosphates; silencing of cytosolic thymidine kinase, which catalyzes the formation of dTMP from thymidine

Genes and Proteins
Gene name	TK1
Gene identifier	7083
Protein name	Thymidine kinase, cytosolic
Protein identifier	P04183
Genetic manipulation/variation	silencing
Gene name	DCTPP1
Gene identifier	79077
Protein name	dCTP pyrophosphatase 1
Protein identifier	Q9H773
Genetic manipulation/variation	mutation
Mutation in protein	knockout