DNTP005684
dCTP
| dNTP Pool | |
|---|---|
| Pool identifier | POOL001426 |
| Members of this Pool |
|
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Martínez-Arribas B, Requena CE, Pérez-Moreno G, Ruíz-Pérez LM, Vidal AE, González-Pacanowska D: DCTPP1 prevents a mutator phenotype through the modulation of dCTP, dTTP and dUTP pools.,
Cell Mol Life Sci, 2020
PubMed PMCID |
| Value | 2.42 | Dimension | pmol/106 cell |
| Error (standard deviation) | 0.96 |
| Data presentation in publication | diagram |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| Cell line/type as in publication | DCTPP1-knockout derivative of HAP1 |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | methanol |
| Remarks to measurement type | Sherman and Fyfe |
| Growth conditions | IMDM supplemented with 10% FBS and antibiotics; cells were transfected with non-targeting siRNA (control) |
| Treatment | |
|---|---|
| Treatment details/effects | lack of functional dCTP pyrophosphatase 1, which hydrolyzes dNTPs to the corresponding nucleoside monophosphates |
| Genes and Proteins | |
|---|---|
| Gene name | DCTPP1 |
| Gene identifier | 79077 |
| Protein name | dCTP pyrophosphatase 1 |
| Protein identifier | Q9H773 |
| Genetic manipulation/variation | mutation |
| Mutation in protein | knockout |