Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001419
Members of this Pool
Members of control pools
POOL001418
Last modified: 10/Sep/2021
Results
Publication
Requena CE, Pérez-Moreno G, Ruiz-Pérez LM, Vidal AE, González-Pacanowska D: The NTP pyrophosphatase DCTPP1 contributes to the homoeostasis and cleansing of the dNTP pool in human cells., Biochem J, 2014

PubMed
Value 9.3
Dimension pmol/106 cell
Error (standard deviation) 0.32
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
CLO Cell line name HeLa cell (CLO:0003684)
Cell line/type as in publication HeLa
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type Sherman and Fyfe
Growth conditions DMEM supplemented with 1×MEM Non-essential Amino Acids solution, L-glutamine, 10% FBS and antibiotics; cells were transfected with DCTPP1 silencing siRNA
Treatment
Treatment details/effects silencing of dCTP pyrophosphatase 1, wich hydrolyzes deoxynucleoside triphosphates to the corresponding nucleoside monophosphates
Genes and Proteins
Gene name DCTPP1
Gene identifier 79077
Protein name dCTP pyrophosphatase 1
Protein identifier Q9H773
Genetic manipulation/variation silencing