DNTP005662
dTTP
dNTP Pool | |
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Pool identifier | POOL001419 |
Members of this Pool |
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Members of control pools |
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POOL001418 |
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Last modified: 10/Sep/2021
Results | |
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Publication |
Requena CE, Pérez-Moreno G, Ruiz-Pérez LM, Vidal AE, González-Pacanowska D: The NTP pyrophosphatase DCTPP1 contributes to the homoeostasis and cleansing of the dNTP pool in human cells.,
Biochem J, 2014
PubMed |
Value | 9.3 | Dimension | pmol/106 cell |
Error (standard deviation) | 0.32 |
Data presentation in publication | diagram |
Source | |
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Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
Sample source | cell line |
CLO Cell line name | HeLa cell (CLO:0003684) |
Cell line/type as in publication | HeLa |
Compartment | whole cell |
Experiment Details | |
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Measurement type | dNTP incorporation assay |
Extraction method | methanol |
Remarks to measurement type | Sherman and Fyfe |
Growth conditions | DMEM supplemented with 1×MEM Non-essential Amino Acids solution, L-glutamine, 10% FBS and antibiotics; cells were transfected with DCTPP1 silencing siRNA |
Treatment | |
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Treatment details/effects | silencing of dCTP pyrophosphatase 1, wich hydrolyzes deoxynucleoside triphosphates to the corresponding nucleoside monophosphates |
Genes and Proteins | |
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Gene name | DCTPP1 |
Gene identifier | 79077 |
Protein name | dCTP pyrophosphatase 1 |
Protein identifier | Q9H773 |
Genetic manipulation/variation | silencing |