DNTP005653
dTTP
| dNTP Pool | |
|---|---|
| This entry does not belong to any pool in the database. |
Last modified: 10/Sep/2021
| Results | |
|---|---|
| Publication |
Nutter LM, Grill SP, Cheng YC: The sources of thymidine nucleotides for virus DNA synthesis in herpes simplex virus type 2-infected cells.,
J Biol Chem, 1985
PubMed |
| Value | 29.2 | Dimension | % |
| Error | Error not provided. |
| Relative compared to | dTTP originating from host DNA |
| Data presentation in publication | numerical |
| Source | |
|---|---|
| Source organism |
human
(Homo sapiens)
Taxonomy 9606 |
| Sample source | cell line |
| CLO Cell line name | HeLa cell (CLO:0003684) |
| Cell line/type as in publication | HeLa |
| Compartment | whole cell |
| Experiment Details | |
|---|---|
| Measurement type | dNTP incorporation assay |
| Extraction method | acid extraction |
| Growth conditions | cells were maintained in RPMI 1640 medium supplemented with 5% fetal calf serum and antibiotics; cells were infected with 3 PFU/cell herpes simplex virus type 2 (HSV-2) for 1 hour, then resuspended in fresh medium containing 0.5 mM hydroxyurea and grown for 12 hours |
| Treatment | |
|---|---|
| Drug/stress type | drug |
| Stress type | viral infection |
| PubChem | 3657 |
| Applied drug | hydroxyurea |
| Treatment details/effects | cells were infected with herpes simplex virus type 2 (HSV-2); cells were treated with hydroxyurea, a ribonucleoside diphosphate reductase inhibitor |
| Genes and Proteins | |
|---|---|
| No genes or proteins manipulated. | |