Results
Publication	Mendel DB, Barkhimer DB, Chen MS: Biochemical basis for increased susceptibility to Cidofovir of herpes simplex viruses with altered or deficient thymidine kinase activity., Antimicrob Agents Chemother, 1995 PubMed PMCID
Value	47.96
Dimension	pmol/106 cell
Error (standard deviation)	1.54
Data presentation in publication	diagram

Source
Source organism	African green monkey (Chlorocebus aethiops) Taxonomy 9534
Sample source	cell line
CLO Cell line name	MA 104 cell (CLO:0007492)
Cell line/type as in publication	MA-104
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	Sherman and Fyfe with minor modifications
Growth conditions	cells were grown in minimum essential medium containing Earle’s salts and supplemented with 10% fetal bovine serum; confluent monolayers of cells were infected with HSV SC16-S1 virus (TK altered mutant) (3 to 5 PFU per cell) for 1 hour, then cells were cultured in fresh medium for 5 hours

Treatment
Drug/stress type	stress
Stress type	viral infection
Treatment details/effects	cells were infected with HSV SC16-S1 virus (TK altered mutant)

Genes and Proteins
Gene name	TK
Gene identifier	Not available.
Protein name	Thymidine kinase
Protein identifier	P06479
Genetic manipulation/variation	mutation in infecting virus
Mutation in protein	TK altered mutant