Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001394
Members of this Pool
Members of control pools
POOL001390
Last modified: 10/Sep/2021
Results
Publication
Mendel DB, Barkhimer DB, Chen MS: Biochemical basis for increased susceptibility to Cidofovir of herpes simplex viruses with altered or deficient thymidine kinase activity., Antimicrob Agents Chemother, 1995

PubMed PMCID
Value 553.08
Dimension pmol/106 cell
Error Error not provided.
Data presentation in publication diagram
Source
Source organism African green monkey (Chlorocebus aethiops)
Taxonomy 9534
Sample source cell line
CLO Cell line name MA 104 cell (CLO:0007492)
Cell line/type as in publication MA-104
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type Sherman and Fyfe with minor modifications
Growth conditions cells were grown in minimum essential medium containing Earle’s salts and supplemented with 10% fetal bovine serum; confluent monolayers of cells were infected with wild-type HSV SC16 virus (3 to 5 PFU per cell) for 1 hour, then cells were cultured in fresh medium for 7 hours
Treatment
Drug/stress type stress
Stress type viral infection
Treatment details/effects cells were infected with wild-type HSV SC16 virus
Genes and Proteins
No genes or proteins manipulated.