DNTP005558
dTTP
dNTP Pool | |
---|---|
Pool identifier | POOL001391 |
Members of this Pool |
|
Members of control pools |
---|
POOL001390 |
|
Last modified: 10/Sep/2021
Results | |
---|---|
Publication |
Mendel DB, Barkhimer DB, Chen MS: Biochemical basis for increased susceptibility to Cidofovir of herpes simplex viruses with altered or deficient thymidine kinase activity.,
Antimicrob Agents Chemother, 1995
PubMed PMCID |
Value | 88.66 | Dimension | pmol/106 cell |
Error | Error not provided. |
Data presentation in publication | diagram |
Source | |
---|---|
Source organism |
African green monkey
(Chlorocebus aethiops)
Taxonomy 9534 |
Sample source | cell line |
CLO Cell line name | MA 104 cell (CLO:0007492) |
Cell line/type as in publication | MA-104 |
Compartment | whole cell |
Experiment Details | |
---|---|
Measurement type | dNTP incorporation assay |
Extraction method | methanol |
Remarks to measurement type | Sherman and Fyfe with minor modifications |
Growth conditions | cells were grown in minimum essential medium containing Earle’s salts and supplemented with 10% fetal bovine serum; confluent monolayers of cells were infected with wild-type HSV SC16 virus (3 to 5 PFU per cell) for 1 hour, then cells were cultured in fresh medium for 1 hour |
Treatment | |
---|---|
Drug/stress type | stress |
Stress type | viral infection |
Treatment details/effects | cells were infected with wild-type HSV SC16 virus |
Genes and Proteins | |
---|---|
No genes or proteins manipulated. | |