Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
Pool identifier POOL001375
Members of this Pool
Members of control pools
POOL001374
Last modified: 10/Sep/2021
Results
Publication
Huang CY, Yagüe-Capilla M, González-Pacanowska D, Chang ZF: Quantitation of deoxynucleoside triphosphates by click reactions., Sci Rep, 2020

PubMed PMCID
Value 8.1
Dimension pmol/106 cell
Error (standard deviation) 2.1
Data presentation in publication numerical
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
CLO Cell line name HCT 116 cell (CLO:0003665)
Cell line/type as in publication HCT 116
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type method article; click reaction method
Growth conditions cells were maintained in McCoy’s 5A with 10% fetal bovine serum and antibiotics; cells were treated with 1 μM doxorubicin for 1 hour, then recovered in regular medium for 8 hours
Treatment
Drug/stress type drug
PubChem 31703
Applied drug doxorubicin
Treatment details/effects cells were treated with doxorubicin, which intercalates between base pairs in the DNA helix, additionally, doxorubicin inhibits topoisomerase II
Genes and Proteins
No genes or proteins manipulated.