Results
Publication	Lee MH, Wang L, Chang ZF: The contribution of mitochondrial thymidylate synthesis in preventing the nuclear genome stress., Nucleic Acids Res, 2014 PubMed PMCID
Value	0.16
Dimension	pmol/106 cell
Error (unspecified)	0
Data presentation in publication	diagram

Source
Source organism	human (Homo sapiens) Taxonomy 9606
Sample source	cell line
CLO Cell line name	IMR-90 cell (CLO:0006951)
Cell line/type as in publication	IMR-90
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	Ferraro
Growth conditions	cells were infected by TK2 shRNA lentivirus, then incubated in DMEM supplemented with 0.1% serum for 10 days, then in DMEM supplemented with 0.1% serum and 5 μM thymidine for 8 hours

Treatment
Drug/stress type	stress
Stress type	starvation
Treatment details/effects	serum deprivation; silencing of mitochondrial Thymidine kinase 2, which phosphorylates thymidine, deoxycytidine, and deoxyuridine in the mitochondrial matrix

Genes and Proteins
Gene name	TK2
Gene identifier	7084
Protein name	Thymidine kinase 2, mitochondrial
Protein identifier	O00142
Genetic manipulation/variation	silencing