Results
Publication	Hu CM, Chang ZF: Synthetic lethality by lentiviral short hairpin RNA silencing of thymidylate kinase and doxorubicin in colon cancer cells regardless of the p53 status., Cancer Res, 2008 PubMed
Value	6.6
Dimension	pmol/106 cell
Error (standard error)	0.8
Data presentation in publication	diagram

Source
Source organism	human (Homo sapiens) Taxonomy 9606
Sample source	cell line
CLO Cell line name	HCT 116 cell (CLO:0003665)
Cell line/type as in publication	HCT-116
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	Sherman and Fyfe
Growth conditions	cells were cultured McCoy’s 5A medium supplemented with 10% fetal bovine serum, and infected with lentiviral TMPK shRNA for 6 hours, then cells were maintained in the medium for 3 days

Treatment
Treatment details/effects	silencing of Thymidylate kinase (TMPK), which catalyzes the formation of dTDP from dTMP

Genes and Proteins
Gene name	DTYMK
Gene identifier	1841
Protein name	Thymidylate kinase
Protein identifier	P23919
Genetic manipulation/variation	silencing