Results
Publication	Ke PY, Kuo YY, Hu CM, Chang ZF: Control of dTTP pool size by anaphase promoting complex/cyclosome is essential for the maintenance of genetic stability., Genes Dev, 2005 PubMed PMCID
Value	5.5
Dimension	pmol/106 cell
Error (standard deviation)	0
Data presentation in publication	diagram

Source
Source organism	mouse (Mus musculus) Taxonomy 10090
Sample source	cell line
CLO Cell line name	NIH-3T3 cell (CLO:0008172)
Cell line/type as in publication	NIH 3T3
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	Sherman and Fyfe
Growth conditions	DMEM with 10% fetal bovine serum and antibiotics (for dNTP pool measurement)

Treatment
Treatment details/effects	overexpression of a nondegradable KEN-box mutant cytosolic Thymidine kinase (hTK1; (dTMP from thymidine); overexpression of a nondegradable D-box mutant Thymidylate kinase (hTMPK; dTDP from dTMP)

Genes and Proteins
Gene name	TK1
Gene identifier	7083
Protein name	Thymidine kinase, cytosolic
Protein identifier	P04183
Genetic manipulation/variation	extrachromosomal expression of mutant protein
Mutation in protein	mutation in a KEN-box motif
Gene name	DTYMK
Gene identifier	1841
Protein name	Thymidylate kinase
Protein identifier	P23919
Genetic manipulation/variation	extrachromosomal expression of mutant protein
Mutation in protein	mutation in a D-box motif (D2)