Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
N/A N/A
Last modified: 10/Sep/2021
Results
Publication
Ke PY, Kuo YY, Hu CM, Chang ZF: Control of dTTP pool size by anaphase promoting complex/cyclosome is essential for the maintenance of genetic stability., Genes Dev, 2005

PubMed PMCID
Value 14.89
Dimension pmol/106 cell
Error (standard deviation) 2.14
Data presentation in publication diagram
Source
Source organism mouse (Mus musculus)
Taxonomy 10090
Sample source cell line
CLO Cell line name NIH-3T3 cell (CLO:0008172)
Cell line/type as in publication NIH 3T3
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type Sherman and Fyfe
Growth conditions DMEM with 10% fetal bovine serum and antibiotics
Treatment
Treatment details/effects overexpression of ATP-binding defective mutant version of Thymidylate kinase (hTMPK)
Genes and Proteins
Gene name DTYMK
Gene identifier 1841
Protein name Thymidylate kinase
Protein identifier P23919
Genetic manipulation/variation extrachromosomal expression of mutant protein
Mutation in protein defective mutation of ATP-binding site (D15R