Thymidine-5'-triphosphate (Pubchem)
dNTP Pool
This entry does not belong to any pool in the database.
Members of control pools
N/A
Last modified: 10/Sep/2021
Results
Publication
Ke PY, Kuo YY, Hu CM, Chang ZF: Control of dTTP pool size by anaphase promoting complex/cyclosome is essential for the maintenance of genetic stability., Genes Dev, 2005

PubMed PMCID
Value 18.56
Dimension pmol/106 cell
Error (standard deviation) 1.41
Data presentation in publication diagram
Source
Source organism human (Homo sapiens)
Taxonomy 9606
Sample source cell line
CLO Cell line name HELA-S3 cell (CLO:0003699)
Cell line/type as in publication HeLa S3
Compartment whole cell
Experiment Details
Measurement type dNTP incorporation assay
Extraction method methanol
Remarks to measurement type Sherman and Fyfe
Growth conditions Cells were arrested by nocodazole treatment for 20 h, followed by replenishment with fresh medium to release cells from the G2/M arrest. Cells were harvested 4 hours after release.
Treatment
Drug/stress type drug
PubChem 4122
Applied drug nocodazole
Treatment details/effects cells were arrested by nocodazole and then released from mitotic block 0h , mitotic progression experiment
Genes and Proteins
No genes or proteins manipulated.