Results
Publication	Ke PY, Kuo YY, Hu CM, Chang ZF: Control of dTTP pool size by anaphase promoting complex/cyclosome is essential for the maintenance of genetic stability., Genes Dev, 2005 PubMed PMCID
Value	53.61
Dimension	pmol/106 cell
Error (standard deviation)	3.74
Data presentation in publication	diagram

Source
Source organism	human (Homo sapiens) Taxonomy 9606
Sample source	cell line
CLO Cell line name	HeLa cell (CLO:0003684)
Cell line/type as in publication	HeLa
Compartment	whole cell

Experiment Details
Measurement type	dNTP incorporation assay
Extraction method	methanol
Remarks to measurement type	Sherman and Fyfe
Growth conditions	cells were transfected with siRNA corresponding to Cdc20, Cdh1 and hTK1, then maintained in DMEM with 10% fetal bovine serum and antibiotics for 48 hours

Treatment
Treatment details/effects	silencing of Cdc20, required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C); silencing of Cdh1, substrate-specific adapter for the APC/C complex; silencing of cytosolic Thymidine kinase (hTK1), which catalyzes formation of dTMP from thymidine

Genes and Proteins
Gene name	CDC20
Gene identifier	991
Protein name	Cell division cycle protein 20 homolog
Protein identifier	Q12834
Genetic manipulation/variation	silencing
Gene name	FZR1
Gene identifier	51343
Protein name	Fizzy-related protein homolog
Protein identifier	Q9UM11
Genetic manipulation/variation	silencing
Gene name	TK1
Gene identifier	7083
Protein name	Thymidine kinase, cytosolic
Protein identifier	P04183
Genetic manipulation/variation	silencing